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Image Search Results
Journal: Cancer Cell International
Article Title: Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
doi: 10.1186/s12935-016-0379-1
Figure Lengend Snippet: Forced expression of TFF3 in cervical cancer cells modulates the expression of malignant progression-related gene markers. a Determination of endogenous expression of TFF3 protein by Western blot in the cervical cancer cells lines CaSki, SiHa, Me180 and Hela and human non-tumor keratinocyte line HaCaT. b Semi-quantitative RT-PCR analysis was used to assess the mRNA levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3 as described in “ ” section. c Western blot analysis was used to assess the protein levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3. d , e Quantitative PCR analysis quantifying the change in expression of various genes associated with malignant progression in SiHa-TFF3/Hela cells. Change in gene expression is expressed as fold difference, respectively. Fold change values are representative of three independent experiments
Article Snippet: Human cervical cancer cell lines SiHa, CaSki, Hela,
Techniques: Expressing, Western Blot, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Gene Expression
Journal: PLoS ONE
Article Title: Suppressor of Cytokine Signaling (SOCS) Genes Are Silenced by DNA Hypermethylation and Histone Deacetylation and Regulate Response to Radiotherapy in Cervical Cancer Cells
doi: 10.1371/journal.pone.0123133
Figure Lengend Snippet: Expression of SOCS1 (A), SOCS3 (B), and SOCS5 (C) gene were examined by qRT-PCR in normal cervix (N Cx) tissue, three normal fibroblast cell lines (CCD-18Co, CCD-18Lu, WI-38) and cervix cancer cell lines (CaSki, HeLa, ME-180, SiHa). Expression level of normal control samples marked as open bar and cervix cancer cell closed bar. Asterisk (*), statistically significant ( p <0.05).
Article Snippet: The human
Techniques: Expressing, Quantitative RT-PCR, Control
Journal: PLoS ONE
Article Title: Suppressor of Cytokine Signaling (SOCS) Genes Are Silenced by DNA Hypermethylation and Histone Deacetylation and Regulate Response to Radiotherapy in Cervical Cancer Cells
doi: 10.1371/journal.pone.0123133
Figure Lengend Snippet: CaSki (A,D), HeLa (B,E), and ME-180 (C,F) cells were treated with 10 μM of 5-Azacytosine (5-Aza) for 72 h (A-C) or infected with control lentivirus (shSCR) or lentivirus expressing shRNA targeting DNMT1 (shDNMT1) (D-F). Knock-down of DNMT1 and SOCS gene expression was examined with qRT-PCR. Asterisk (*), statistically significant ( p <0.05).
Article Snippet: The human
Techniques: Infection, Control, Expressing, shRNA, Knockdown, Gene Expression, Quantitative RT-PCR